Skip to Content
Merck
CN
All Photos(2)

Documents

Safety Information

RAB0476

Sigma-Aldrich

Human Tumor Necrosis Factor α ELISA Kit

for serum, plasma, cell culture supernatants and urine

Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
TNF-alpha
UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 24.58-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNF(7124)

General description

The Human TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-α in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TNF-α

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Tumor Necrosis Factor α ELISA Kit has been used to measure the concentration of TNF-α in pre-adipocyte growth medium, blood sample and in human bronchial epithelial cell (BEAS 2B) culture supernatantand normal human keratinocytes (NHK) cell culture supernatant.

Biochem/physiol Actions

Tumor necrosis factor- α (TNF-α) helps in regulating immune response and it acts as a proinflammatory cytokine. TNF-α is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Overexpression of the protein has been observed in serum from oral lichen planus (OLP) patients. Mutation in the gene promotor increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

WGK

WGK 3

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Human leukocyte antigen class I and II alleles in non-Hodgkin lymphoma etiology
Wang SS, et al.
Blood, 115(23), 4820-4823 (2010)
Association between TNF alpha-308 G/A polymorphism and oral lichen planus (OLP): a meta-analysis
Zhou Yand Vieira AR
Journal of Applied Oral Science : Revista FOB, 26 (2018)
Tanshinone II is a potent candidate for treatment of lipopolysaccharide-induced acute lung injury in rat model
Li L, et al.
Oncology Letters, 15(2), 2550-2554 (2018)
Apelin attenuates oxidative stress in human adipocytes.
Than A
The Journal of Biological Chemistry, 289(6), 3763-3774 (2014)
Luca Fusaro et al.
Frontiers in bioengineering and biotechnology, 8, 281-281 (2020-04-23)
Cardiovascular diseases represent the leading cause of death in developed countries. Modern surgical methods show poor efficiency in the substitution of small-diameter arteries (<6 mm). Due to the difference in mechanical properties between the native artery and the substitute, the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service