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Merck
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RAB0435

Human SOST ELISA Kit

for serum, plasma, cell culture supernatants and urine

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... SOST(50964)
Input:
sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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Quality Level

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 40 pg/mL
standard curve range: 40.96-10000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SOST(50964)

General description

The Human SOST (also known as sclerostin) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SOST in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human SOST

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Pamela S Hinton et al.
Bone, 96, 85-90 (2016-10-21)
We previously reported that 12months of resistance training (RT, 2×/wk, N=19) or jump training (JUMP, 3×/wk, N=19) increased whole body and lumbar spine BMD and increased serum bone formation markers relative to resorption in physically active (≥4h/wk) men (mean age:
Joshua F Baker et al.
The Journal of rheumatology, 44(12), 1777-1785 (2017-09-17)
Rheumatoid arthritis (RA) is associated with muscle loss, osteoporosis, and fracture. We examined associations between skeletal muscle mass, strength, and quality and trabecular and cortical bone deficits in patients with RA and healthy controls. Participants, ages 18-75 years, completed whole-body
Jennifer Dekker et al.
Pediatric exercise science, 29(4), 504-512 (2017-05-23)
This study examined resting levels of catabolic and anabolic osteokines related to Wnt signaling and their responses to a single bout of plyometric exercise in child and adolescent females. Fourteen premenarcheal girls [10.5 (1.8) y old] and 12 postmenarcheal adolescent girls
Chi-Chien Niu et al.
BMC musculoskeletal disorders, 18(1), 61-61 (2017-02-06)
Wnt signaling plays an important role in development and maintenance of many organs and tissues. The most-studied secreted Wnt inhibitors are sclerostin (SOST), Dickkopf-related protein 1 (DKK-1), and secreted frizzled related protein 1 (SFRP-1) which play important roles in bone
Masanori Koide et al.
Scientific reports, 10(1), 13751-13751 (2020-08-15)
Bone tissues have trabecular bone with a high bone turnover and cortical bone with a low turnover. The mechanisms by which the turnover rate of these bone tissues is determined remain unclear. Osteocytes secrete sclerostin, a Wnt/β-catenin signaling antagonist, and

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