RAB0418
Human Ubiquitin+1 ELISA Kit
for serum, plasma, cell culture supernatant and urine
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41116158
NACRES:
NA.77
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... RPS27A(6233)
Related Categories
General description
The Human Ubiquitin+1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ubiquitin+1 in serum, plasma, cell culture supernatants and urine.
The RPS27A (ribosomal protein S27a) gene is mapped to human chromosome 2p16.1. The protein complex encoded contains ubiquitin at the N-terminus and a C-terminal ribosomal protein S27a.
The RPS27A (ribosomal protein S27a) gene is mapped to human chromosome 2p16.1. The protein complex encoded contains ubiquitin at the N-terminus and a C-terminal ribosomal protein S27a.
Immunogen
Recombinant Human Ubiquitin
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Ribosomal protein S27a (RPS27a), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression and inhibits apoptosis of leukemia cells. Overexpression of RPS27A results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients. Elevated expression of UBCEP-1, which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis. RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression.
The ribosomal protein S27a (Rps27a) possesses two main functions, it is responsible for ribosome formation and takes part in post translational modification of proteins. DNA damage elevates the RPS27A gene expression. The RPS27A gene is upregulated in chronic myelogenous leukemia and colorectal cancer. It is known to stimulate cell cycle by inducing cellular checkpoints through p53 and cell proliferation and prevent apoptosis in leukemia cells.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Kit Components Also Available Separately
Product No.
Description
SDS
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Inverse correlation of ribosomal protein S27A and multifunctional protein YB-1 in hepatocellular carcinoma.
Gunasekaran V P and Ganeshan M
Clinical Biochemistry, 47(13-14), 1262-1264 (2014)
A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer.
Ko Y, et al.
International Journal of Molecular Medicine, 15(2), 183-196 (2005)
Overexpression of RPS27a contributes to enhanced chemoresistance of CML cells to imatinib by the transactivated STAT3.
Wang H, et al.
Oncotarget, 7(14), 18638-18638 (2016)
Identification and expression of MMSA-8, and its clinical significance in multiple myeloma.
He R, et al.
Oncology Reports, 37(6), 3235-3243 (2017)
DNA damage stress induces the expression of ribosomal protein S27a gene in a p53-dependent manner.
Nosrati N, et al.
Gene, 559(1), 44-51 (2015)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service