R9894
Ras human
Wild type, recombinant, expressed in E. coli, buffered aqueous glycerol solution
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Synonym(s):
H-Ras, rH-RAS, WT
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥80% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
28 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... RASGEF1B(153020)
General description
Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.
Application
Human Ras has been used as a negative control in tubulin overlay assay.
Biochem/physiol Actions
Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.
Packaging
Package size based on protein content
Physical form
Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Test (2006)
Inactivation of Ras GTPase-activating proteins promotes unrestrained activity of wild-type Ras in human liver cancer
Calvisi DF, et al.
Journal of Hepatology, 54(2), 311-311 (2011)
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Methods in Enzymology (2006)
J Tucker et al.
The EMBO journal, 5(6), 1351-1358 (1986-06-01)
v-Ha-ras encoded p21 protein (p21V), the cellular c-Ha-ras encoded protein (p21C) and its T24 mutant form p21T were produced in Escherichia coli under the control of the tac promoter. Large amounts of the authentic proteins in a soluble form can
Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
Jean-Luc D
The Journal of Biological Chemistry (1997)
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