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Merck
CN

R8005

RPMI-1640 Medium

Hybri-Max™, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

Synonym(s):

RPMI 1640 Culture Medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Quality Level

500

form

powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Preparation Note

Formulated to contain 16.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000500482
0000500482
25C331
25C331
0000435197

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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