R7904
Anti-Rab5 antibody, Mouse monoclonal
clone Rab5-65, purified from hybridoma cell culture
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Synonym(s):
Anti-RAS-associated Protein RAB5A
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Rab5-65, monoclonal
form
buffered aqueous solution
mol wt
~24 kDa
species reactivity
human, cat, bovine, mouse, rat
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK
indirect ELISA: suitable
microarray: suitable
western blot: suitable
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)
General description
Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.
Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.
Immunogen
synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.
Application
Anti-Rab5 antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- co-immunoprecipitation assay
- western blotting
- immunofluorescence
Biochem/physiol Actions
Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).
Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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