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Safety Information

R7020

Sigma-Aldrich

Transcript RNA Markers 0.2-10 kb

for RNA electrophoresis

Synonym(s):

RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker

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Select a Size

500 ML
¥434.58
1 L
¥700.66
6 X 500 ML
¥2,085.93
24 X 500 ML
¥7,822.27

¥434.58

Available to ship on2025年4月16日Details

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Select a Size

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500 ML
¥434.58
1 L
¥700.66
6 X 500 ML
¥2,085.93
24 X 500 ML
¥7,822.27

About This Item

UNSPSC Code:
41105337
NACRES:
NA.25

¥434.58

Available to ship on2025年4月16日Details

grade

Molecular Biology

Quality Level

300

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

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Show Differences

1 of 4

This Item
116807541281644927
3-Chloroperbenzoic acid ≤77%

273031

3-Chloroperbenzoic acid

3-Phenylbutyric acid 98%

116807

3-Phenylbutyric acid

Methaneseleninic acid 95%

541281

Methaneseleninic acid

3-Methylpyrazole-5-carboxylic acid 97%

644927

3-Methylpyrazole-5-carboxylic acid

reaction suitability

reagent type: oxidant

reaction suitability

-

reaction suitability

-

reaction suitability

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

-

mp

69-71 °C (lit.)

mp

35-38 °C (lit.)

mp

128-132 °C (lit.)

mp

236-240 °C (lit.)

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

form

solid

form

solid

form

-

form

solid

General description

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.[1]

Components

Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ8407
    SLCQ8407
    0000342041
    0000342041
    SLCP7867

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    Wright SA, et al.
    Plant Pathology, 62, 982-992 (2013)
    Magda Dudek et al.
    Nucleic acids research, 48(14), 7786-7800 (2020-06-26)
    Marine flavobacteria possess dedicated Polysaccharide Utilization Loci (PULs) enabling efficient degradation of a variety of algal polysaccharides. The expression of these PULs is tightly controlled by the presence of the substrate, yet details on the regulatory mechanisms are still lacking.

    Articles

    Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

    Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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