R6878
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse
~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-phospho-Rb (pSer795)
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About This Item
Conjugate:
unconjugated
Clone:
RB-10, monoclonal
Application:
immunohistochemistry
indirect ELISA
microarray
western blot
indirect ELISA
microarray
western blot
Species reactivity:
mouse, rat, human, bovine, hamster
Citations:
5
Technique(s):
immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
Uniprot accession no.:
Product Name
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse, ~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RB-10, monoclonal
form
buffered aqueous solution
mol wt
antigen 110 kDa
species reactivity
mouse, rat, human, bovine, hamster
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer795)
Quality Level
Gene Information
human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)
Application
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse has been used in:
- immunoblotting
- immunocytochemistry
- immunofluorescence
- fluorescence-activated cell sorting (FACS) assays
- enzyme linked immunosorbent assay (ELISA)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Biochem/physiol Actions
Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS795 is required to inactivate Rb imposed growth suppression.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.
Immunogen
synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer795) of human retinoblastoma protein conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
Regulatory Information
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Cellular mechanisms of tumour suppression by the retinoblastoma gene
Burkhart DL and Sage J
Nature Reviews. Cancer, 8(9), 671-671 (2008)
Karen J Meaburn et al.
Aging cell, 6(2), 139-153 (2007-02-06)
A number of diseases associated with specific tissue degeneration and premature aging have mutations in the nuclear envelope proteins A-type lamins or emerin. Those diseases with A-type lamin mutation are inclusively termed laminopathies. Due to various hypothetical roles of nuclear
The E2F1-3 transcription factors are essential for cellular proliferation
Wu L, et al.
Nature, 414(6862), 457-457 (2001)
Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells
Ohmine S, et al.
Oncology Reports, 39(4), 1725-1730 (2018)
Yan Li et al.
Molecular medicine reports, 14(3), 2222-2230 (2016-07-20)
Ginsenoside Rg3 (Rg3) is one of the primary constituents isolated from ginseng, and has been found to exhibit cytotoxic effects against cancer cells. The present study aimed to investigate the effects of Rg3 on human multiple myeloma cell proliferation and
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