R6504
RPMI-1640 Medium
With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
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About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
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General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.
Application
RPMI-1640 Medium is used for the following applications:
- used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
- used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
- used for cell isolation and culture
- used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
- used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
- used for the preparation of antifungal agents
Quantity
Formulated to contain 10.4 grams of powder per liter of medium.
Reconstitution
Supplement with 2.0 g/L sodium bicarbonate.
recommended
Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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R Roubenoff et al.
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Invasive fungal infections caused by Aspergillus (A.) and Mucorales species still represent life-threatening diseases in immunocompromised individuals, and deeper knowledge about fungal interactions with elements of innate immunity, such as complement and platelets, appears essential for optimized therapy. Previous studies
Manuel Cuenca-Estrella et al.
Antimicrobial agents and chemotherapy, 46(11), 3644-3647 (2002-10-18)
A two-laboratory study was performed to evaluate the correlation between the NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. A panel of 109 bloodstream isolates was tested against amphotericin B, flucytosine, fluconazole, and itraconazole. Overall, the
Articles
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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