R5886
RPMI-1640 Medium
HEPES modification, without ʟ-glutamine, sterile liquid, suitable for cell culture
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Synonym(s):
Roswell Park Memorial Institute 1640 medium
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
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General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.
Application
RPMI-1640 Medium has been used:
- to culture human lymphoblastoid cell line cells
- prostate cancer cells
- monocytes from human blood
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Flash Point(F)
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Flash Point(C)
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Regulatory Information
动植物来源培养基
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