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R5158

Sigma-Aldrich

RPMI-1640 Medium

HEPES modification with ʟ-glutamine, sterile liquid, suitable for cell culture

Synonym(s):

RPMI culture medium, Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.71

product name

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

>7.2

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

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