R4655
Anti-Rab27 (N-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
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All Photos(2)
Synonym(s):
Anti-RAB27A, member RAS oncogene family
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~25 kDa
species reactivity
human, rat
concentration
~1.5 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extract of human A431 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAB27A(5873)
rat ... Rab27a(50645)
General description
Rab27 (Ras-related protein) is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab27 is widely expressed in non-neuronal regulated secretory cells, including exocrine, endocrine, ovarian, and hematopoietic cells.
Rab27A (Ras-related protein) belongs to the Rab family of small GTPase proteins. It is located on chromosome 15q21.
Immunogen
synthetic peptide corresponding to amino acid residues 45-57 of human RAB27A, with an added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse Rab27A and by 2 amino acids in human RAB27B.
Application
Anti-Rab27 (N-terminal) antibody has been used in immunoblotting, immunofluorescence.
Biochem/physiol Actions
In humans, Rab27A (Ras-related protein) plays important roles in the development of cancers. It is considered as a prognostic biomarker for CRC (colorectal cancer) patients. It may participate in the transfer of protein and signal transduction mediated by GTPase. Rab27 mutation is linked with Griscelli syndrome type 2. It is also responsible for the innate immune resistance against invading microorganisms.
Rab27 (Ras-related protein) regulates the exocytosis of these cell-specific store organelles, using multiple organelle-specific effector proteins, such as granuphilin and melanophilin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
related product
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Rab27a was identified as a prognostic biomaker by mRNA profiling, correlated with malignant progression and subtype preference in gliomas
Wang H, et al.
PLoS ONE, 9(2) (2014)
High Rab27A expression indicates favorable prognosis in CRC
Shi C, et al.
Diagnostic Pathology, 10:68 (2015)
Vectorial release of hepatitis E virus in polarized human hepatocytes
Capelli N, et al.
Journal of virology, 93(4), e01207-e01218 (2019)
Bong Hwan Sung et al.
Nature communications, 6, 7164-7164 (2015-05-15)
Directional cell movement through tissues is critical for multiple biological processes and requires maintenance of polarity in the face of complex environmental cues. Here we use intravital imaging to demonstrate that secretion of exosomes from late endosomes is required for
Hironori Higashio et al.
Scientific reports, 6, 22539-22539 (2016-03-05)
Mast cell degranulation is regulated by the small guanosine triphosphatases (GTPases) Rab27a and Rab27b, which have distinct and opposing roles: Rab27b acts as a positive regulator through its effector protein Munc13-4, a non-neuronal isoform of the vesicle-priming Munc13 family of
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