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Merck
CN

R4130

RPMI-1640 Medium

HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.75
MDL number:
MFCD00217820

Key Documents

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Product Name

RPMI-1640 Medium, HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Preparation Note

Formulated to contain 16.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000461166
0000461164
0000461164
25B345
25B345

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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