R1780
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, sterile liquid, suitable for cell culture, suitable for isotope labeling for cell culture applications
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Synonym(s):
Roswell Park Memorial Institute 1640 medium
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L
shipped in
ambient
storage temp.
2-8°C
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General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Reconstitution
Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).
also commonly purchased with this product
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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