R129
Monoclonal Anti-Ryanodine Receptor antibody produced in mouse
clone 34C, ascites fluid
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Synonym(s):
Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
34C, monoclonal
mol wt
antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)
species reactivity
human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep
technique(s)
direct immunofluorescence: suitable
immunohistochemistry (frozen sections): 1:1,000
immunoprecipitation (IP): suitable
western blot: 1:5,000
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RYR1(6261) , RYR2(6262)
mouse ... Ryr1(20190) , Ryr2(20191)
rat ... Ryr1(114207) , Ryr2(84025)
General description
The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.
The ryanodine receptor (RyR) is the channel responsible for the release of Ca2+ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca2+ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
Immunogen
partially purified chicken pectoral muscle ryanodine receptors (RyR)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.
Physical form
Purified IgG1 antibody containing 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Janet E Jeffrey et al.
Arthritis research & therapy, 9(6), R129-R129 (2007-12-22)
This study investigated the release of prostaglandin E2 (PGE2) from cartilage following an impact load in vitro and the possible chondroprotective effect of cyclooxygenase-2 (COX-2) inhibition using non-steroidal anti-inflammatory drugs (NSAIDs). Explants of human articular cartilage were subjected to a
Michele Scorzeto et al.
PloS one, 8(10), e74919-e74919 (2013-10-08)
Mitochondrial calcium handling and its relation with calcium released from sarcoplasmic reticulum (SR) in muscle tissue are subject of lively debate. In this study we aimed to clarify how the SR determines mitochondrial calcium handling using dCASQ-null mice which lack
Jeffrey J Kelu et al.
Developmental biology, 438(1), 57-68 (2018-03-27)
During the development of the early spinal circuitry in zebrafish, spontaneous Ca2+ transients in the primary motor neurons (PMNs) are reported to transform from being slow and uncorrelated, to being rapid, synchronized and patterned. In this study, we demonstrated that
Vanessa Schartner et al.
Acta neuropathologica, 133(4), 517-533 (2016-12-25)
Muscle contraction upon nerve stimulation relies on excitation-contraction coupling (ECC) to promote the rapid and generalized release of calcium within myofibers. In skeletal muscle, ECC is performed by the direct coupling of a voltage-gated L-type Ca2+ channel (dihydropyridine receptor; DHPR)
Katja Anttila et al.
Acta histochemica et cytochemica, 40(2), 35-41 (2007-06-20)
In this study, the fiber type specificity of dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs) in different rat limb muscles was investigated. Western blot and histochemical analyses provided for the first time evidence that the expression of both receptors correlates
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