R1158
Ribonuclease Inhibitor
recombinant, expressed in E. coli, 20-40 units/μL
Synonym(s):
RNAse inhibitor
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About This Item
recombinant
expressed in E. coli
Quality Level
grade
for molecular biology
form
buffered aqueous solution
mol wt
~50 kDa
concentration
20-40 units/μL
shipped in
dry ice
storage temp.
−20°C
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General description
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).
Application
Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:
- cDNA synthesis from mRNA
- reverse transcriptase polymerase chain reaction (RT-PCR)
- in vitro transcription and translation
- mRNA protection from degradation
- formaldehyde cross-linked lncRNA purification
- RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Biochem/physiol Actions
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Components
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Unit Definition
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Physical form
Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.
Other Notes
- Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
- This product is for R&D use only, not for drug, household, or other uses.
related product
Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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