R1145
RPMI-1640 Medium
Without ʟ-glutamine, folic acid and sodium bicarbonate, sterile liquid, suitable for cell culture, 10x
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Synonym(s):
Roswell Park Memorial Institute 1640 medium
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
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General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Reconstitution
Supplement with 0.3 g/L L-glutamine, 2.0 g/L sodium bicarbonate, 0.001 g/L folic acid at 1×.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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