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Safety Information

R0884

Sigma-Aldrich

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):

RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219

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1 MG
CN¥741.91
5 MG
CN¥2,375.11
10 MG
CN¥3,354.73
20 MG
CN¥5,748.88

CN¥741.91

Estimated to ship onApril 21, 2025Details

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1 MG
CN¥741.91
5 MG
CN¥2,375.11
10 MG
CN¥3,354.73
20 MG
CN¥5,748.88

About This Item

CAS Number:
9014-24-8
Enzyme Commission number:
2.7.7.6 (BRENDA, IUBMB)
MDL number:
MFCD00132197
UNSPSC Code:
12352204

CN¥741.91

Estimated to ship onApril 21, 2025Details

recombinant

expressed in E. coli

grade

Molecular Biology

form

buffered aqueous solution

mol wt

98.8 kDa

concentration

10,000-50,000 U/mL

UniProt accession no.

P00573

foreign activity

DNase and RNase, none detected

storage temp.

−20°C

Gene Information

bacteriophage T7 ... T7p07(1261050)

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Piperonylamine 97%

P49503

Piperonylamine

Piperonal 99%

P49104

Piperonal

Piperonyl alcohol 98%

P49406

Piperonyl alcohol

2-Aminomethyl-1,4-benzodioxane 97%

678848

2-Aminomethyl-1,4-benzodioxane

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

density

1.214 g/mL at 25 °C (lit.)

density

-

density

-

density

1.1679 g/mL at 25 °C

refractive index

n20/D 1.564 (lit.)

refractive index

-

refractive index

-

refractive index

n20/D 1.5594

bp

138-139 °C/13 mmHg (lit.)

bp

264 °C (lit.)

bp

-

bp

105-110 °C/3 mmHg

General description

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Components

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Unit Definition

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Analysis Note

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    SLBQ8964V

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    Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
    M Chamberlin et al.
    The Journal of biological chemistry, 248(6), 2235-2244 (1973-03-25)
    V D Axelrod et al.
    Biochemistry, 24(21), 5716-5723 (1985-10-08)
    RNA synthesis by T7 RNA polymerase or SP6 RNA polymerase is 100-1000 times more sensitive to the presence of the 3'-deoxyribonucleoside 5'-triphosphate chain terminators than is RNA synthesis by Escherichia coli RNA polymerase or Q beta replicase. These ribonucleotide analogues
    Pemphigus vulgaris is characterized by low IgG reactivities to specific self-antigens along with high IgG reactivity to desmoglein 3.
    Fatta L
    Immunology, 143(3), 374-380 (2014)
    Albert Weixlbaumer et al.
    Cell, 152(3), 431-441 (2013-02-05)
    Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause
    Tokiko Watanabe et al.
    Journal of virology, 87(9), 5239-5254 (2013-03-02)
    The 1918 pandemic influenza virus was the most devastating infectious agent in human history, causing fatal pneumonia and an estimated 20 to 50 million deaths worldwide. Previous studies indicated a prominent role of the hemagglutinin (HA) gene in efficient replication
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