R0503
Reactive Red 120−Agarose
saline suspension, Type 3000-CL
Synonym(s):
Reactive Red Agarose, Red 120-Agarose, Red Agarose
Product Name
Reactive Red 120−Agarose, saline suspension, Type 3000-CL
biological source
plant
type
Type 3000-CL
form
saline suspension
extent of labeling
≥3 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
suitability
suitable for chromatography
storage temp.
2-8°C
Quality Level
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Application
Reactive Red 120-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Red 120-agarose has been used to study wheat quality breeding as well as to provide strong evidence that purified human P-glycoprotein (Pgp) functions as an ATP-dependent drug transporter.
Physical form
Suspension in 0.5 M NaCl containing preservative
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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S Takeda et al.
Journal of biochemistry, 114(5), 684-690 (1993-11-01)
A particulate fraction consisting of heavy organelles such as nuclei and mitochondria was prepared from Ehrlich ascites tumor cells. From this fraction we have purified a GTP-binding protein with a molecular mass of 33 kDa (MTG33) by guanidine hydrochloride extraction
Galit Yehezkel et al.
The Journal of biological chemistry, 281(9), 5938-5946 (2005-12-16)
In this study, we addressed the presence and location of nucleotide-binding sites in the voltage-dependent anion channel (VDAC). VDAC bound to reactive red 120-agarose, from which it was eluted by ATP, less effectively by ADP and AMP, but not by
C F Barroga et al.
Protein science : a publication of the Protein Society, 5(6), 1093-1099 (1996-06-01)
The nucleotide-binding component of the high-affinity ribose transport system of Escherichia coli, RbsA, was overproduced from a T7-7 expression vector, and the protein was purified. Biochemical analyses of the purified protein indicated that the ATP analogues, 5'-FSBA and 8-azido ATP
Gregory A Bannikov et al.
American journal of veterinary research, 68(9), 995-1004 (2007-09-04)
To characterize and purify covalent complexes of matrix metalloproteinase-9 (MMP-9) and haptoglobin released by bovine granulocytes in vitro. Blood samples obtained from healthy cows and cows with acute and chronic inflammation to obtain WBCs and sera. WBCs were isolated by
S V Ambudkar et al.
Methods in enzymology, 292, 492-504 (1998-08-26)
Human Pgp from the vinblastine-resistant cell line, KB-V1, can be purified by sequential conventional chromatography on DEAE-sepharose CL-6B resin followed by a wheat germ agglutinin column. By including glycerol (osmolyte protectant) and lipid during the solubilization and chromatography procedures most
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