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PRKE

Sigma-Aldrich

Enterokinase Removal Kit

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Synonym(s):
PRK-E
UNSPSC Code:
12352202
eCl@ss:
32160410
NACRES:
NA.56

shipped in

wet ice

storage temp.

2-8°C

General description

Kit is designed to remove all enterokinase (as judged by antigenic or enzymatic activity assays). This is accomplished by binding with immobilized rabbit antibodies to calf intestine enterokinase followed by spin filtration.

Application

For removal of enterokinase by immunoaffinity from fusion protein cleavage reaction mixtures. 100 μl of a 50% slurry of the anti-enterokinase-agarose can remove at least 7 μg of bovine enterokinase or 1 μg of recombinant catalytic subunit.

Preparation Note

Dilute required amount of 20X Wash Buffer 1:20 in distilled water.

Kit Components Only

Product No.
Description

  • 20× Wash buffer 4 mL

  • Disposable spin filters 10 ea

Kit Components Also Available Separately

Product No.
Description
SDS

  • Anti-Enterokinase-agarose 1.5 mL

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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