PP0100
Trypsin Profile IGD Kit
Synonym(s):
In gel digestion kit, Proteomics grade Trypsin, TPCK treated
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About This Item
NACRES:
NA.56
UNSPSC Code:
12352200
General description
The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.
Application
Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.
Features and Benefits
- Faster destaining than with alternative in-gel digest kits
- Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
- Compatible with Coomassie, SYPRO™ Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
- Resulting samples are ready for analysis by MALDI-MS or HPLC-MS
Legal Information
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Kit Components Only
Product No.
Description
- Destaining Solution Reconstituted to 75
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
35.6 °F - closed cup
flash_point_c
2.0 °C - closed cup
wgk
WGK 2
Regulatory Information
危险化学品
低风险生物材料
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Articles
Protein modifications are crucial for disease study. Analysis methods are key.
One of the most important aspects of our ultra-pure MALDI matrix substances is their ability to dissolve rapidly and completely; a brief vortex mixing is typically sufficient.
SILAM for quantitative proteomics of liver Akt1/PKBa after burn injury.
Lu XM, et al.
International Journal of Molecular Medicine, 29, 461-461 (2012)
Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PP0100-1KT | 04061835574728 |