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Sigma-Aldrich

Trypsin Profile IGD Kit

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Synonym(s):
In gel digestion kit, Proteomics grade Trypsin, TPCK treated
UNSPSC Code:
12352200
NACRES:
NA.56

usage

 kit sufficient for ≤100 applications

Quality Level

200

storage temp.

2-8°C

Related Categories

General description

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Application

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.

Features and Benefits

  • Faster destaining than with alternative in-gel digest kits
  • Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
  • Compatible with Coomassie, SYPRO® Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
  • Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a registered trademark of Life Technologies

Kit Components Only

Product No.
Description
  • Destaining Solution Reconstituted to 75

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 2

Flash Point(F)

35.6 °F

Flash Point(C)

2.0 °C

Regulatory Information

危险化学品
动植物源性产品

Certificates of Analysis (COA)

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Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
SILAM for quantitative proteomics of liver Akt1/PKBa after burn injury.
Lu XM, et al.
International Journal of Molecular Medicine, 29, 461-461 (2012)
X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
Shuo Han et al.
Cell death & disease, 12(11), 1070-1070 (2021-11-12)
Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA
Irene Riz et al.
Methods in molecular biology (Clifton, N.J.), 699, 431-447 (2010-12-01)
In vivo biotinylation tagging, based on a method in which a protein of interest is tagged with a peptide that is biotinylated in vivo by coexpression of Escherichia coli BirA biotin ligase, has been successfully used for the isolation of
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