PLA0227
Rabbit anti-BRD4 Antibody, Affinity Purified
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Synonym(s):
CAP, HUNK1, HUNKI, MCAP, Protein HUNK1, bromodomain-containing 4, chromosome-associated protein
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
grade
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species reactivity
human, mouse
concentration
1 mg/mL
technique(s)
immunohistochemistry: 1:1,000-1:5,000
immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000-1:10,000
accession no.
NP_490597.1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
rabbit ... BRD4(23476)
General description
Bromodomain-containing protein 4 (BRD4) belongs to the bromodomain and extra-terminal (BET) family. The protein has two bromodomains (BD1 and BD2) connected by a long interdomain linker.
Immunogen
The epitope recognized by PLA0227 maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).
Biochem/physiol Actions
Bromodomain-containing protein 4 (BRD4) works as a reader of epigenetic marks and is involved in chromatin remodeling and transcriptional regulation. Therefore, it is a target for oncology, inflammation and cardiovascular disease. This gene is upregulated in hepatocellular carcinoma and is associated with poor prognosis. BRD4 is also associated with melanoma, hepatocellular carcinoma, multiple myeloma, Burkitt′s lymphoma, acute myeloid leukemia and breast cancer. BRD4 connects cell cycle and transcription, works as a scaffold for transcription factors and bookmarks active genes during mitosis. It interacts with acetylated histone-3 and histone-4 tails and helps in maintaining chromatin architecture. Also, it regulates transcription elongation via phosphorylation of RNA polymerase II.
Physical form
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Other Notes
Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Segmental Isotope Labelling of an Individual Bromodomain of a Tandem Domain BRD4 Using Sortase A.
Williams F P, et al.
PLoS ONE, 11(4), e0154607-e0154607 (2016)
BRD4 promotes tumor growth and epithelial-mesenchymal transition in hepatocellular carcinoma.
Zhang P, et al.
International Journal of Immunopathology and Pharmacology, 28(1), 36-44 (2015)
High level of BRD4 promotes non-small cell lung cancer progression.
Liao Y F, et al.
Oncotarget, 7(8), 9491-9491 (2016)
Shun-Hong Luo et al.
Cell death & disease, 14(11), 760-760 (2023-11-23)
Lipid metabolism is the key to ferroptosis susceptibility. However, little is known about the underlying mechanisms in osteosarcoma cells. Functional restriction of bromodomain-containing protein 4 (BRD4) reduced the susceptibility to erastin-induced ferroptosis of osteosarcoma cells both in vitro and in
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