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Safety Information
PFM50
GenElute™ Five-Minute Plasmid Miniprep Kit
sufficient for 50 purifications
Synonym(s):
GenElute™ Plasmid Miniprep Kit, Gen Elute
Select a Size
CN¥1,498.09
Available to ship onApril 25, 2025Details
Select a Size
About This Item
CN¥1,498.09
Available to ship onApril 25, 2025Details
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usage
sufficient for 50 purifications
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1 of 4
This Item | 911410 | 911429 | 911399 |
|---|---|---|---|
| form powder | form powder | form powder | form powder or chunks |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt PEG average Mn 5,000 | mol wt PEG average Mn 5,000 (by NMR), PLGA average Mn 10,000 (by NMR) | mol wt PEG average Mn 5,000 (by NMR), PLGA average Mn 5,000 (by NMR) | mol wt PEG average Mn 2,000 (by NMR), PLGA average Mn 10,000 (by NMR) |
| composition lactide:glycolide 50:50 | composition - | composition - | composition - |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 100 |
General description
Recombinant E. coli overnight culture in LB (Luria-Bertani) broth is treated briefly (1-2 minutes) with a lysis reagent to effect rapid cell lysis and RNA degradation, without prior removal of culture medium. A binding solution is then mixed with the lysate, and DNA is captured on a silica-based binding column. Impurities are removed by a wash solution and bound plasmid DNA is then eluted in Tris buffer or water, ready for immediate use in various downstream applications. Typical yields of high copy plasmids are 2-6 μg from 400 μL of overnight culture in LB broth.
The GenElute Five-Minute Plasmid Miniprep Kits utilize a novel lysis procedure that eliminates the need to pellet cells from an overnight culture, remove growth media or clear a neutralized lysate. 400 μl of culture is added to a 2 mL collection tube and cells are lysed for 1−2 minutes. A Binding Solution is then added and the entire mixture is passed through a binding column. After washing the column to remove trace contaminants, the plasmid DNA is eluted in 10 mM Tris HCl, pH 8.5. Molecular biology water may be substituted as an elution reagent.
Application
Features and Benefits
- No pelleting cells or clearing lysates
- Binding column works with any standard laboratory vacuum manifold
- Up to 5 μg of plasmid DNA or enough for 15 sequencing reactions
- DNA suitable for immediate capillary DNA sequencing
Other Notes
Legal Information
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1C - STOT SE 3
Target Organs
Central nervous system
Supplementary Hazards
Storage Class Code
3 - Flammable liquids
Flash Point(F)
69.8 °F
Flash Point(C)
21 °C
Regulatory Information
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