PURedit^® Cas9 Protein

Functional Genomics; Target Validation; Genome Editing

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

Recombinant PURedit Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes PURedit Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

PURedit is a registered trademark of Merck KGaA, Darmstadt, Germany

Each kit consists of:

• one vial of PURedit Cas9 Recombinant Protein
• one vial of PURedit Transfection Buffer
• one vial of Reconstitution Buffer
• one vial of Dilution Buffer

Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION^® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)

Lyophilized PURedit Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.