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Key Documents

Safety Information

PCRISPR004

Sigma-Aldrich

Mouse CRISPR Brie Knockout Library

Synonym(s):

CRISPR Brie Library, CRISPR Library for Mice, Mouse Knockout Library

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100 G
CN¥811.31
500 G
CN¥3,853.70

CN¥811.31

Available to ship onApril 27, 2025Details

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100 G
CN¥811.31
500 G
CN¥3,853.70

About This Item

UNSPSC Code:
41105904
NACRES:
NE.02

CN¥811.31

Available to ship onApril 27, 2025Details

Request a Bulk Order

packaging

pkg of 5 vials (5x200µL aliquots )

concentration

≥5x108 VP/ml (via p24 Assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

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抗-淀粉样蛋白β肽（MOAB-2），pan抗体，克隆6C3 clone 6C3, from mouse

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ABN240

Anti-Amyloid, β 1-40, aβ Antibody

Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody clone M98, from rabbit

MABN640

Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody

抗-淀粉样蛋白β42抗体，克隆G2-13 clone G2-13, from mouse

MABN13

抗-淀粉样蛋白β42抗体，克隆G2-13

clone

6C3, monoclonal

clone

polyclonal

clone

M98, monoclonal

clone

G2-13, monoclonal

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Application

  • Functional Genomics/Target Validation
  • Unbiased wholed genome forward genetic screening
  • Validated positive and negative controls
  • Set up and optimization of screen assay

Features and Benefits

  • Focus on your research, and we will generate your lentivirus screening library.
  • Use CRISPR nucleases to knockout protein-coding genes to assess their function.
  • Mouse Genes targeted 19,674
  • Compact library of ~ four gRNAs per gene (78,637 total)
  • Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.

Principle

In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.

Legal Information

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

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Certificates of Analysis (COA)

Lot/Batch Number

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John G Doench et al.
Nature biotechnology, 34(2), 184-191 (2016-01-19)
CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target

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