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P9582

Sigma-Aldrich

Polyinosinic–polycytidylic acid potassium salt

with buffer salts, TLR ligand tested

Synonym(s):

Poly (I:C), Poly(I) • Poly(C)

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About This Item

CAS Number:
31852-29-6
MDL number:
MFCD00131983
UNSPSC Code:
41106305
NACRES:
NA.51

Quality Level

300

Assay

≥99% (less than 1% free nucleotides, TLC)

form

lyophilized powder

composition

Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)

storage condition

desiccated

storage temp.

−20°C

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Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Packaging

Package size based on polynucleotide content

Other Notes

Double-stranded homopolymer.

Preparation Note

The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How stable is Product P9582, Polyinosinic-polycytidylic acid potassium salt, in solution?

    RNA and synthetic RNA analogues will be very stable in aqueous solution as long as the solutions are not contaminated with RNase.

  6. Should Polyinosinic-polycytidylic acid potassium salt Product P9582 or P1038 be used for cell activation?

    For cell activation, synthetic double-stranded RNA is widely used as ligand of Toll-like receptor 3 (TLR3) in place of what is supposed to be the natural ligand (viral dsRNA). The double stranded RNA homopolymer, i.e. Poly(I):Poly(C), Prod. No. P9582 can be used for this application.  P1038 is a single-stranded RNA and will not activate TLR3.

  7. What is the difference between Polyinosinic-polycytidylic acid potassium salt Products P9582, P1038, P1530 and P0913?     

    Prod No. P9582 as well as Prod. Nos. P1530 and P0913 are double-stranded with one strand of Poly(I) and one strand of Poly(C).  Prod. No. P1038 is a single-stranded RNA analog with C and I randomly making up the polynucelotide strand.  Double-stranded Prod. No. P9582 is a potassium salt while double-stranded Prod. Nos. P0913 and P1038 are sodium salts.  Prod. No. P0913 is gamma-irradiated.  None of the other products have been gamma-irradiated.  Unlike the other products, only approximately 10% of the  total mass of Prod. No. P9582 is actually polynucleotide.  The remaining 90% is buffer salts as sodium chloride and sodium phosphate.  

  8. What is the molecular weight of Product P9582, Polyinosinic-polycytidylic acid potassium salt?

    The molecular weight may vary from lot to lot with a range from 90,000 to 1,400,000 with an average of 200,000 to 500,000.     

  9. Why is the total mass of Product P9582, Polyinosinic-polycytidylic acid potassium salt, in my bottle much greater than the designated package size?

    The package size is based on the polynucleotide content.  However, since the polynucleotide is supplied with sodium chloride and sodium phosphate buffer salts, its mass represented by the package size is approximately 10% of the total mass present in the bottle.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses. Evidence from investigations in
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