P9290
Proteinase K–Agarose from Tritirachium album
lyophilized powder
Synonym(s):
Proteinase K-Agarose
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About This Item
biological source
microbial (T.album
T. ALBUM)
Quality Level
form
lyophilized powder
extent of labeling
30-140 units per g solid
technique(s)
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
swelling
1 g swells to ~5 mL
storage temp.
−20°C
Application
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Biochem/physiol Actions
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Features and Benefits
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Physical form
Lyophilized powder stabilized with lactose
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Shai Eyal et al.
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To type Klebsiella pneumonia through methods including pulse-field gel electrophoresis (PFGE) in combination with multilocus sequence typing. Four selected different EPs, referring to the Standard Operating Procedure of PulseNet China, were used. The single colony of Klebsiella pneumonia was quantified
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