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CN

P9273

Pro-Phe-Arg-7-amido-4-methylcoumarin

≥95%

Synonym(s):

PFR-Nmec

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About This Item

Empirical Formula (Hill Notation):
C30H37N7O5 · C2H4O2
CAS Number:
65147-21-9
Molecular Weight:
635.71
UNSPSC Code:
12352204
PubChem Substance ID:
24899025
NACRES:
NA.32

Key Documents

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SMILES string

NC(NCCC[C@@H](C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)NC([C@H](CC3=CC=CC=C3)NC(C4NCCC4)=O)=O)=N.CC([O-])=O

InChI

1S/C30H37N7O5.ClH/c1-18-15-26(38)42-25-17-20(11-12-21(18)25)35-28(40)23(10-6-14-34-30(31)32)36-29(41)24(16-19-7-3-2-4-8-19)37-27(39)22-9-5-13-33-22;/h2-4,7-8,11-12,15,17,22-24,33H,5-6,9-10,13-14,16H2,1H3,(H,35,40)(H,36,41)(H,37,39)(H4,31,32,34);1H

InChI key

QYFPUTDFLKFOBA-UHFFFAOYSA-N

assay

≥95%

form

powder

composition

Peptide content, ≥65%

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

Quality Level

200

Application

Pro-Phe-Arg-7-amido-4-methylcoumarin has been used as fluorogenic substrate:
  • in kinetic experiments to measure the enzymatic activity of factor XII
  • to measure the activity of generated plasma kallikrein using Spectramax plate reader
  • to determine the cathepsin A (CatA) inhibitor selectivity against various proteases using Tecan Safire 2 plate reader

General description

A fluorogenic substrate for pancreatic and urinary kallikreins.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000497297
0000497297
0000475358
0000475358
0000436882

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H Wang et al.
Biomedical chromatography : BMC, 10(3), 139-143 (1996-05-01)
A method was developed for the purification of kallikrein from human urine. The procedure consisted of three steps: ultradialysis, diethyl-(2-hydroxypropyl) aminoethyl (QAE) ion exchange radial flow membrane chromatography and affinity chromatography on aprotinin agarose. It is simple and suitable for
H Kato et al.
Journal of biochemistry, 87(4), 1127-1132 (1980-04-01)
Kallikrein in human urine was measured using a fluorogenic peptide substrate, proly-phenyl-alanyl-arginine-4-methylcoumaryl-7-amide (Pro-Phe-Arg-MCA). Using 20 microliters of normal urine, kallikrein activity was quantitatively assayed by incubation with a final concentration of 10(-4) M Pro-Phe-Arg-MCA at 37 degrees C for 90
L Li et al.
Clinical and experimental immunology, 120(2), 261-266 (2000-05-03)
Chemical catalysis, an effector mechanism utilized by fully assembled antibodies, can also be mediated by the isolated antibody subunits. Because trace amounts of free light chains (L chains) are present in IgG preparations, a detailed study was undertaken to identify
Y Kitamura et al.
Biochimica et biophysica acta, 1387(1-2), 153-164 (1998-09-28)
The culture medium in which prehatching embryos of the frog, Rana pirica, were cultured (hatching medium) solubilized the vitelline coat (VC) of unfertilized eggs and contained molecules reactive to antibodies (anti-UVS.2) against the Xenopus hatching enzyme (HE). The hydrolyzing activity
K Uchida et al.
Biochimica et biophysica acta, 614(2), 501-510 (1980-08-07)
Kallikrein (EC 3.4.21.8) was purified from rat stomach by column chromatography on p-aminobenzamidine-Sepharose, DEAE-Sephadex A-50 and Sephadex G-150 and by isoelectric focusing, measuring its activities to hydrolyse L-prolyl-L-phenylalanyl-L-arginine-4-methyl-coumaryl-7-amide and to release kinin from heat-treated rat plasma. the purified stomach kallikrein
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