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P9269

Millipore

Protein A–Agarose

lyophilized powder

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MDL number:
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

Quality Level

extent of labeling

~2 mg per mL

matrix

4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥10 mg/mL binding capacity (human IgG)

storage temp.

−20°C

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General description

This Protein A-Agarose product is a conjugate of Protein A to CNBr-activated agarose without an intermediate spacer arm.

Application

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Quantity

Swelling: 1 g swells to approx. 4 ml

Physical form

Supplied as lyophilized powder stabilized with lactose.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immune regulation in murine schistosomiasis japonica: inhibition of in vitro antigen- and mitogen-induced cellular responses by splenocyte culture supernatants and by purified fractions from serum of chronically infected mice.
K S Garb et al.
Journal of immunology (Baltimore, Md. : 1950), 129(6), 2752-2758 (1982-12-01)
Carl F Fortin et al.
International immunology, 22(8), 637-649 (2010-05-27)
Polymorphonuclear neutrophils (PMNs) are the first line of defense against invading organisms in humans; in addition, PMNs contribute to the linking of innate and adaptive immunity. To fulfill their biological behavior, PMNs utilize an arsenal of proteolytic enzymes, including members
Fenghua Hu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(5), 1262-1269 (2008-02-01)
Myelin-derived Nogo-A protein limits axonal growth after CNS injury. One domain binds to the Nogo-66 receptor to inhibit axonal outgrowth, whereas a second domain, Amino-Nogo, inhibits axonal outgrowth and cell adhesion through unknown mechanisms. Here, we show that Amino-Nogo inhibition
Steffen Krauss et al.
Journal of virology, 83(24), 12854-12870 (2009-10-09)
Our previous work has shown that efficient evasion from type I interferon responses by human cytomegalovirus (hCMV) requires expression of the 72-kDa immediate-early 1 (IE1) protein. It has been suggested that IE1 inhibits interferon signaling through intranuclear sequestration of the
Stefania Trazzi et al.
PloS one, 4(6), e5832-e5832 (2009-06-09)
CENP-C is a fundamental component of functional centromeres. The elucidation of its structure-function relationship with centromeric DNA and other kinetochore proteins is critical to the understanding of centromere assembly. CENP-C carries two regions, the central and the C-terminal domains, both

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