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P9172

Sigma-Aldrich

Pyronin Y

for NA electrophoresis

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Synonym(s):
Pyronin Y, Pyronin G, Pyronin J
Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12352200
PubChem Substance ID:
57654569
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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Application

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
  • staining of CD34+ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride
Darzynkiewicz Z and Carter SP
Cancer research, 48(5) (1988)
Staining of RNA after polyacrylamide gel electrophoresis.
K Marcinka
Methods in cell biology, 10, 49-68 (1975-01-01)
Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities
Affer M, et al.
Journal of Oncology, 2011 (2011)
Transcriptional profiling of mammary gland side population cells
Behbod F, et al.
Stem Cells, 24(4), 1065-1074 (2006)
Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres
Wang J, et al.
Blood, 124(22), 3237-3240 (2014)
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