P8147
Platelet-Derived Growth Factor from human platelets
PDGF, lyophilized powder, suitable for cell culture
Synonym(s):
PDGF
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About This Item
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biological source
human
Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.5-10.0 ng/mL EC50
quality
endotoxin tested
mol wt
glycoprotein 28-31 kDa
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... PDGFA(5154) , PDGFB(5155)
General description
Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers: PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. The active form of PDGF exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.
Application
PDGF is a chemoattractant for fibroblasts, smooth muscle cells, monocytes and neutrophils. Also, it acts on fibroblasts and other cells that initiate healing and tissue restoration.
Biochem/physiol Actions
Platelet-derived growth factor (PDGF), not only functions as a growth factor, but also participates in chemotaxis for triggering cell movement. Elevated levels of placental PDGF is observed during the late first and third-trimester pregnancies. PDGF is associated with wound healing. PDGFRα (PDGF receptor α ) phosphorylation, mediated by PDGFA activates pathways associated with cancer. PDGFA has been indicated to induce growth and metastasis in human hepatocellular carcinoma. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signaling and is known to promote tumor angiogenesis in vivo. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia.
Other Notes
Isolated from human platelets, predominantly containing two covalently linked subunits (A and B).
Physical form
Lyophilized from a 0.2-μm filtered solution in 40% acetonitrile plus 0.1% TFA
Analysis Note
The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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J W Assender et al.
The Biochemical journal, 288 ( Pt 2), 527-532 (1992-12-01)
The effects on cellular proliferation and Ca2+ mobilization of analogues of cyclic AMP (cAMP) and cyclic GMP (cGMP) and of agents that elevate the intracellular concentrations of cyclic nucleotides were compared in closely similar preparations of first-passage rabbit aortic vascular
Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro
Flood MR, et al.
Theriogenology, 39(4), 823-833 (1993)
Overexpression of platelet-derived growth factor-BB increases tumor pericyte content via stromal-derived factor-alpha CXCR4 axis
Song N, et al.
Cancer Research, 69(15), 6057-6064 (2009)
Shiki Fujino et al.
Molecular cancer research : MCR, 19(5), 812-822 (2021-02-14)
Recently developed molecularly targeted therapies such as EGFR inhibitors have notably improved the prognosis of patients with cancer. However, patients with KRAS and BRAF mutations do not currently benefit from these therapies. Here, we aimed to examine potential effects of
Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis
Kohannim O, et al.
Case Reports in Genetics, 2011 (2011)
Articles
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
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