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P7405

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt >300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Synonym(s):

PDL

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About This Item

Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
27964-99-4
MDL number:
MFCD00131934
UNSPSC Code:
12352202
PubChem Substance ID:
24898848
NACRES:
NA.75

product name

Poly-D-lysine hydrobromide, mol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

biological source

synthetic (chemical)

Quality Level

200

sterility

γ-irradiated

product line

BioReagent
BioXtra

form

lyophilized powder

mol wt

>300,000

packaging

pkg of 5 mg

concentration

0.016—0.032 mmol lysine

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

solubility

H2O: soluble 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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General description

Poly-D-Lysine Hydrobromide (PDL) is a cationic polymer composed of lysine residues with an associated hydrobromide molecule per lysine unit. The presence of the hydrobromide molecule introduces a distinctive property to the poly-D-lysine structure allowing the poly-D-lysine to adopt a crystalline form, rendering it soluble in aqueous solutions.

Application

Poly-D-lysine hydrobromide has been used :
  • in preparing the surface of coverslips for cell attachment
  • in coating glass-bottom dishes for live cell imaging of primary cortical neuronal cells
  • in coating 24-well plates for the isolation of primary astrocytes to study the impact of melanoma-derived exosomes on the reprogramming of stromal cells within the metastatic microenvironment
  • for coating 16-well chamber slides to study the effects of maternal immune activation on behavioral impairments and alterations in cytokine and synaptic protein expression in the cerebellum
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-D-Lysine Hydrobromide (PDL) is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. PDL increases the number of positively charged cell binding sites post-absorption to the culture surface. PDL coating promotes the attachment of cells to solid surfaces that usually grow in suspension.
Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

This product has a molecular weight >300,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy
Hu X, et al.
Oxidative Medicine and Cellular Longevity (2021)
Maternal Immune Activation Causes Behavioral Impairments and Altered Cerebellar Cytokine and Synaptic Protein Expression
Pendyala G, et al.
Neuropsychopharmacology, 42, 1435?1446-1435?1446 (2017)
On the interaction of polystyrene latices and poly(l-lysine): II. Characterization of the particle-polymer System
Corry W.D
Journal of Colloid and Interface Science, 63(1), 151-160 (1978)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner
PLoS ONE, 17(7), e0260173-e0260173 (2022)
CRISPR-Mediated Activation of ?V Integrin Subtypes Promotes Neuronal Differentiation of Neuroblastoma Neuro2a Cells
Riccardi S, et al.
Frontiers in genome editing, 4 (2022)
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Articles

Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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