P7181
Polyethylene glycol solution
Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma
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grade
Hybri-Max™
Quality Level
sterility
0.2 μm filtered
product line
BioReagent
form
solution
mol wt
average mol wt 1,450
concentration
50 % (w/v)
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
SMILES string
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
Related Categories
General description
Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.
Application
Polyethylene glycol solution has been used in the hybridization of the following:
- irradiated camel fibroblast cells with A23 hamster cells
- mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
- rabbit splenocytes and cell line 240E-1
Recommended for use in a normal fusion protocol requiring 50% PEG.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Biochem/physiol Actions
Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.
Packaging
Packaged in sealed ampules under nitrogen.
Other Notes
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.
Reconstitution
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
Flash Point(F)
212.0 °F
Flash Point(C)
100 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Perelman PL, et al.
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