Anti-PRMT1 (TK-16) antibody produced in rabbit

Protein arginine methyl transferase 1 (PRMT1) is a 361 amino acid protein; its splicing variants differ at the N-terminus, giving rise to three isoforms of 343, 361, and 347 amino acids (variants 1, 2, and 3 respectively).

The PRMT1 gene is mapped on the human chromosome at 19q13.33.

Anti-PRMT1 (Protein Arginine Methyl Transferase 1) (TK-16) recognizes PRMT1 (approx. 42 kDa) from human, rat, mouse, monkey, and hamster origin.

synthetic peptide corresponding to amino acids 309-324 of rat PRMT1 conjugated to KLH via an N-terminal added cysteine residue. This sequence is conserved in human and rat.

Anti-PRMT1 (TK-16) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Protein arginine methyl transferase (PRMT) family of enzymes catalyzes the methylation of arginine during post translational modification. These enzymes transfer the methyl group from S-adenosyl-L-methionine to the guanidino nitrogen atoms of an arginine residue. PRMTs are divided in two major types, I and II. Both types catalyze the formation of monomethylarginine, but differs in that Type I (including PRMT1, 3, 4, and 6) catalyzes the formation of asymmetric dimethylarginine, whereas type II (PRMT5) catalyzes the formation of symmetric dimethylarginine. Proteins involved in the metabolism of RNA like 68-kDa Src-associated substrate in mitosis (Sam68) are substrates for PRMT1.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilu-tions should be discarded if not used within 12 hours.

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