P6246
Anti-PABP antibody, Mouse monoclonal
clone 10E10, purified from hybridoma cell culture
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Synonym(s):
Anti-Poly(A)-Binding Protein
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
10E10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~69 kDa
species reactivity
hamster, canine, bovine, Xenopus, human, monkey
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using whole cell extracts of HEK 293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PABPC1(26986)
Related Categories
General description
Monoclonal Anti-PABP (mouse IgG1 isotype) is derived from the 10E10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with recombinant human PABP. Poly(A) binding protein (PABP) is highly conserved between different organisms especially at the amino-terminal region of the protein that contains four RNA-binding domains (RBDs).
Poly(A) binding protein (PABP) possesses RNA recognition motifs (RRMs), a proline-rich region and a PABP C-terminal domain. The gene encoding this protein is localized on human chromosome 8q22.3.
Immunogen
recombinant human PABP.
Application
Monoclonal Anti-PABP antibody produced in mouse has been used for immunofluorescence.
Monoclonal Anti-PABP antibody produced in mouse has been used in:
- Immunoblotting
- Immunostaining
- Immunoprecipitation
- Enzyme linked immunosorbent assay(ELISA)
- Immunocytochemistry
Biochem/physiol Actions
Poly(A) binding protein (PABP) has a role in mRNA translation and stability. It modulates gene expression. The protein binds to the poly(A) tail, bridges the ends of mRNA and forms a closed loop. This might help in translation initiation by promoting ribosome recruitment.
Poly(A) binding protein (PABP) is found in excess, relative to the amount of cytoplasmic poly(A) (three fold) and binds to its poly(A) target at high affinity (Kd of 7nM). The eukaryotic initiation factor, EIF4F complex, interacts with PABP indirectly via the PAIP-1 protein (PABP-interacting protein-1). As a consequence, an interaction between the 5′ and 3′ ends of mRNA occurs. This proximity between the mRNA ends contributes to its stability and enhances translation, since terminating ribosomes may start translation again.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Poly(A) Binding Protein 1 Enhances Cap-Independent Translation Initiation of Neurovirulence Factor from Avian Herpesvirus
Abdessamad T
PLoS ONE (2014)
Hun-Way Hwang et al.
Cell reports, 15(2), 423-435 (2016-04-07)
Accurate and precise annotation of 3' UTRs is critical for understanding how mRNAs are regulated by microRNAs (miRNAs) and RNA-binding proteins (RBPs). Here, we describe a method, poly(A) binding protein-mediated mRNA 3' end retrieval by crosslinking immunoprecipitation (PAPERCLIP), that shows
BTG2 bridges PABPC1 RNA-binding domains and CAF1 deadenylase to control cell proliferation
Stupfler B, et al.
Nature Communications, 7, 10811-10811 (2016)
Poly (A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression
Brook M and Gray NK
Genome Biology, 4(7), 223-223 (2003)
RNA UK 2012: The role of mammalian poly(A)-binding proteins in co-ordinating mRNA turnover
Brook M and Gray NK
Biochemical Society Transactions, 40(Pt 4), 856-856 (2012)
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