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P5994

Sigma-Aldrich

Phosphatase substrate

40 mg tablets

Synonym(s):

4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

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About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
EC Number:
224-246-5
MDL number:
MFCD00066288
UNSPSC Code:
12352204
PubChem Substance ID:
24898723
NACRES:
NA.54

Quality Level

200

form

tablet

concentration

36-44 mg/tablet

impurities

p-nitrophenol, essentially free

solubility

H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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General description

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

Application

Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Quantity

Contains 40 mg substrate per tablet

Preparation Note

Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
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International journal of nanomedicine, 12(11), 7483-7483 (2017)
Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength
Henriksen K, et al.
PLoS ONE, 6(11), e27482-e27482 (2011)
Elucidating human phosphatase-substrate networks
Li X, et al.
Science Signaling, 6(275), rs10-rs10 (2013)
Tetsuya Harakuni et al.
Infection and immunity, 73(9), 5654-5665 (2005-08-23)
Noninvasive mucosal vaccines are attractive alternatives to parenteral vaccines. Although the conjugation of vaccine antigens with the B subunit of cholera toxin (CTB) is one of the most promising strategies for vaccine delivery to mucosal immune systems, the molecule cannot
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α-Tricalcium phosphate (α-TCP) is widely used as a reactant in calcium phosphate cements. This work aims at doping α-TCP with silicon with a twofold objective. On the one hand, to study the effect of Si addition on the stability and
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