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P5431

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

aseptically filled, lyophilized powder, ≥2 units/mg solid

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Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

sterility

aseptically filled

form

lyophilized powder

specific activity

≥2 units/mg solid

storage temp.

2-8°C

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Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Physical form

Lyophilized powder containing lactose

Preparation Note

Prepared from P 3026

comparable product

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Joan Ho-Huu et al.
BMC evolutionary biology, 12, 195-195 (2012-10-03)
Gene duplications are a molecular mechanism potentially mediating generation of functional novelty. However, the probabilities of maintenance and functional divergence of duplicated genes are shaped by selective pressures acting on gene copies immediately after the duplication event. The ratio of
S S Ahmed et al.
Genetics and molecular research : GMR, 11(3), 3091-3104 (2012-09-26)
Molecular chaperones of plasmid pBI121 carrying CaMV35S promoter and a nucleotide sequence of plasmid pBI221 were inserted into plasmid pCAMBIA2300 to construct an intermediate vector: pVBG2307. This novel vector pVBG2307 contains a greatly expanded multiple cloning site with an adjacent
Zamen Ben Romdhane et al.
Applied biochemistry and biotechnology, 169(1), 215-227 (2012-11-28)
The aim of the present study was to investigate pectinases production by CT1 mutant of Penicillium occitanis on glucose based media. Two main groups of pectinases were followed: lyases (pectin and pectate lyases) and hydrolases (polygalacturonases and polymethylgalacturonases). When cultivated
Peerapat Roongsattham et al.
BMC plant biology, 12, 150-150 (2012-08-28)
Cell separation that occurs during fleshy fruit abscission and dry fruit dehiscence facilitates seed dispersal, the final stage of plant reproductive development. While our understanding of the evolutionary context of cell separation is limited mainly to the eudicot model systems
Isabelle Benoit et al.
BMC genomics, 13, 321-321 (2012-07-21)
Pectins are diverse and very complex biomolecules and their structure depends on the plant species and tissue. It was previously shown that derivatives of pectic polymers and oligosaccharides from pectins have positive effects on human health. To obtain specific pectic

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