P5374
Anti-Podoplanin antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-Aggrus, Anti-GP36, Anti-Glycoprotein, 36-KD, Anti-OTS8, Anti-T1α gene, Anti-T1A, Anti-T1A2
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~38 kDa
species reactivity
human, rat
concentration
~1 mg/mL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using heat retrieved, human skin sections
western blot (chemiluminescent): 5-10 μg/mL using human HEK-293 or rat NRK cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PDPN(10630)
rat ... Pdpn(54320)
General description
Podoplanin (PDPN) is a transmembrane sialoglycoprotein. It consists of 162 amino acids and is part of the type-1 transmembrane sialomucin-like glycoprotein family. The protein is expressed in various tissues. The PDPN gene is localized on human chromosome 1p36.21.
Podoplanin is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. Podoplanin, also named E11 antigen, is present in normal, inflammatory and neoplastic lymphatic capillary endothelium. It is expressed selectively by dermal microvascular lymphatic endothelial cells but not by blood vascular endothelial cells.
Immunogen
synthetic peptide corresponding to amino acids 24-40 of human podoplanin with an N-terminal added cysteine, conjugated to KLH.
Application
Anti-Podoplanin antibody produced in rabbit has been used in immunoblotting.
Anti-Podoplanin antibody produced in rabbit has been used in immunohistochemistry.
Biochem/physiol Actions
Podoplanin (PDPN) is expressed in many cancers. In cancerous cells, it induces platelet aggregation and epithelial-mesenchymal transition. The protein is an immunohistochemical marker of lymphatic vessels. Podoplanin also has a role in embryonic development.
Podoplanin appears to play an important role in the maintenance of podocyte foot processes, and hence, glomerular permeability. It is detectable in human angiosarcomas, lymphangiomas and in Kaposi′s sarcomas. Experiments involving depletion of T1a/podoplanin in mice indicate a critical regulatory role for it in formation of the lymphatic vasculature.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide and 1% bovine serum albumin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
The multifaceted role of podoplanin expression in hepatocellular carcinoma.
Cioca A
European Journal of Histochemistry (2017)
Podoplanin associates with adverse postoperative prognosis of patients with clear cell renal cell carcinoma
Yu Xia
Cancer Science (2016)
Podoplanin Expression in Cancer-associated Fibroblasts Predicts Poor Prognosis in Patients with Squamous Cell Carcinoma of the Lung
Yohei Y
Anticancer Research (2017)
Abstract 877: Genetic variation in podoplanin in relation to glioma risk and outcome
Kristen N
Cancer Research (2011)
The potential role of podoplanin in tumour invasion
Wicki A and Christofori G
British Journal of Cancer, 96(1), 1-1 (2007)
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