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P5117

Sigma-Aldrich

Anti-Peripherin antibody, Mouse monoclonal

clone 8G2, purified from hybridoma cell culture

Synonym(s):

Anti-NEF4, Anti-PRPH1

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About This Item

MDL number:
MFCD01633973
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

8G2, monoclonal

form

buffered aqueous solution

species reactivity

rat, bovine, pig, mouse, rabbit, feline, human

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1.0 μg/mL using total cell extract from rat PC12 cells

isotype

IgG1

UniProt accession no.

P41219

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PRPH(5630)
mouse ... Prph(19132)
rat ... Prph(24688)

General description

Monoclonal Anti-Peripherin (mouse IgG1 isotype) is derived from the 8G2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the full-length recombinant rat peripherin. Peripherin was originally identified as a neuronal intermediate filament protein-(IF) is thought to be expressed only in the peripheral nervous system (PNS).
Peripherin is an intermediate filament protein. It is expressed in peripheral and enteric neurons. The gene encoding this protein is localized on rat chromosome 7.

Immunogen

full-length recombinant rat peripherin.

Application

Anti-Peripherin antibody, Mouse monoclonal has been used in:
  • immunohistochemistry
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunofluorescence staining

Biochem/physiol Actions

Peripherin is upregulated during pheochromocytoma PC12 cell differentiation. Later studies have detected peripherin also in certain central nervous system (CNS) neurons.
eripherin has been linked with axonal extension and regeneration of nerve fibers. It also has a role in neurite outgrowth propagation. Peripherin is a substrate of protein kinase B/Akt. Phosphorylation of this protein is induced after nerve injury.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Regulatory Information

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Suppression of Extensive Neurofilament Phosphorylation Rescues alpha-Internexin/Peripherin-Overexpressing PC12 Cells from Neuronal Cell Death
Lee WC, et al.
PLoS ONE, 7(8), e43883-e43883 (2012)
Rania Magadmi et al.
Frontiers in cellular neuroscience, 13, 262-262 (2019-07-06)
Neuroimmune interactions are important in the pathophysiology of many chronic inflammatory diseases, particularly those associated with alterations in sensory processing and pain. Mast cells and sensory neuron nerve endings are found in areas of the body exposed to the external
Identification of peripherin as a Akt substrate in neurons.
Konishi H
The Journal of Biological Chemistry (2007)
New insights into peripherin expression in cochlear neurons.
Lallemend F
Neuroscience (2007)
Diana Tavares-Ferreira et al.
Biological psychiatry, 91(1), 129-140 (2020-12-15)
There are clinically relevant sex differences in acute and chronic pain mechanisms, but we are only beginning to understand their mechanistic basis. Transcriptome analyses of rodent whole dorsal root ganglion (DRG) have revealed sex differences, mostly in immune cells. We
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