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Safety Information

P4789

Sigma-Aldrich

Protease from Bacillus sp.

Type XXVII, lyophilized powder, 7-14 units/mg solid

Synonym(s):

Nagarse

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About This Item

Enzyme Commission number:
EC Number:
UNSPSC Code:
12352204

type

Type XXVII

form

lyophilized powder

specific activity

7-14 units/mg solid

purified by

crystallization

foreign activity

DNase, RNase, essentially free

storage temp.

−20°C

Application

Protease from Bacillus sp. has been used for enzymatic antigen retrieval during immunohistochemical studies. It has also been used in protease treatment for the isolation of mitochondria from muscle specimens.

Biochem/physiol Actions

Protease is widely used in various industries such as the leather industry, food industry, and pharmaceutical industry. It is used in laundry detergents to remove protein-based stains from clothes. In addition, protease also helps in the wound healing process by removing necrotic materials. It also finds application in contact-lens enzyme cleaners and enzymatic deriders. Nargase, a protease isolated from Bacillus sp. is widely used for isolation of interfibrillar (IFM) mitochondria.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin BPN′.

comparable product

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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A Dionigi et al.
American journal of clinical pathology, 114(1), 111-122 (2000-07-08)
Ovarian metastases from colorectal carcinoma frequently mimic primary ovarian carcinomas. The present study was performed to identify possible criteria helpful in differential diagnosis. Twenty-three ovarian metastases from colorectal carcinomas and 23 primary ovarian carcinomas were evaluated clinicopathologically and immunostained with
Gizelda T B Casella et al.
Journal of neuroscience methods, 139(1), 1-11 (2004-09-08)
Methods that facilitate the accurate counting of specific neural cell types would be of substantial value in evaluating the efficacy of treatments applied to spinal cord injury. This report describes reliable procedures for identification of neurons, oligodendrocytes, astrocytes, endothelial cells
L Bakkman et al.
Acta physiologica (Oxford, England), 190(3), 243-251 (2007-05-25)
To investigate if training during hypoxia (H) improves the adaptation of muscle oxidative function compared with normoxic (N) training performed at the same relative intensity. Eight untrained volunteers performed one-legged cycle training during 4 weeks in a low-pressure chamber. One
Nagarse treatment of cardiac subsarcolemmal and interfibrillar mitochondria accounts for inaccurate quantification of proteins
Koncsos G, et al.
Journal of Molecular and Cellular Cardiology, 120, 5-6 (2018)
L H Lash et al.
Toxicology, 130(1), 1-15 (1998-12-10)
Selective permeabilization of plasma membranes with digitonin produced separation of cytosolic and mitochondrial compartments of proximal tubular (PT) and distal tubular (DT) cells from a rat kidney. Subcellular distributions of several intracellular glutathione (GSH)-dependent enzymes were similar in the two

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