P4789
Protease from Bacillus sp.
Type XXVII, lyophilized powder, 7-14 units/mg solid
Synonym(s):
Nagarse
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type
Type XXVII
form
lyophilized powder
specific activity
7-14 units/mg solid
purified by
crystallization
foreign activity
DNase, RNase, essentially free
storage temp.
−20°C
Application
Protease from Bacillus sp. has been used for enzymatic antigen retrieval during immunohistochemical studies. It has also been used in protease treatment for the isolation of mitochondria from muscle specimens.
Biochem/physiol Actions
Protease is widely used in various industries such as the leather industry, food industry, and pharmaceutical industry. It is used in laundry detergents to remove protein-based stains from clothes. In addition, protease also helps in the wound healing process by removing necrotic materials. It also finds application in contact-lens enzyme cleaners and enzymatic deriders. Nargase, a protease isolated from Bacillus sp. is widely used for isolation of interfibrillar (IFM) mitochondria.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin BPN′.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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A Dionigi et al.
American journal of clinical pathology, 114(1), 111-122 (2000-07-08)
Ovarian metastases from colorectal carcinoma frequently mimic primary ovarian carcinomas. The present study was performed to identify possible criteria helpful in differential diagnosis. Twenty-three ovarian metastases from colorectal carcinomas and 23 primary ovarian carcinomas were evaluated clinicopathologically and immunostained with
Gizelda T B Casella et al.
Journal of neuroscience methods, 139(1), 1-11 (2004-09-08)
Methods that facilitate the accurate counting of specific neural cell types would be of substantial value in evaluating the efficacy of treatments applied to spinal cord injury. This report describes reliable procedures for identification of neurons, oligodendrocytes, astrocytes, endothelial cells
Nagarse treatment of cardiac subsarcolemmal and interfibrillar mitochondria accounts for inaccurate quantification of proteins
Koncsos G, et al.
Journal of Molecular and Cellular Cardiology, 120, 5-6 (2018)
Linda Bakkman et al.
Obesity facts, 3(6), 371-375 (2011-01-05)
The extent of weight gain varies among individuals despite equal calorie overconsumption. Furthermore, weight gain is often less than expected from energy excess. This suggests differences in metabolic efficiency and basal metabolism. Since mitochondrial uncoupling accounts for a substantial portion
L H Lash et al.
Toxicology, 130(1), 1-15 (1998-12-10)
Selective permeabilization of plasma membranes with digitonin produced separation of cytosolic and mitochondrial compartments of proximal tubular (PT) and distal tubular (DT) cells from a rat kidney. Subcellular distributions of several intracellular glutathione (GSH)-dependent enzymes were similar in the two
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