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P4664

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

chromogenic, tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein:
3912045
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898491
NACRES:
NA.83

Product Name

o-Phenylenediamine dihydrochloride, tablet, 15 mg substrate per tablet

Quality Level

200

form

tablet

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used:
  • as a substrate in conventional capture enzyme-linked immunosorbent assay (ELISA) for recombinant hemagglutinin (rHA) protein detection
  • as a substrate for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin G (IgG) antibodies by ELISA
  • as a colorimetric reagent specific for horseradish peroxidase (HRP) activity assessment in an ELISA protocol using SARS-CoV-2 antigens for S protein

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000390834
0000381937
0000381936
0000310684
0000310689

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E Werle et al.
The Journal of rheumatology, 28(3), 595-600 (2001-04-12)
To determine the prevalence and potential diagnostic relevance of autoantibodies against serotonin, thromboplastin, and ganglioside Gm1 in patients with fibromyalgia syndrome (FM). Sera from 203 patients with FM and 64 pain-free control subjects were analyzed with enzyme immunoassays. Clinical and
Hepatitis B virus transgenic severe combined immunodeficient mouse model of acute and chronic liver disease.
Mark A Feitelson et al.
Methods in molecular medicine, 96, 269-287 (2004-02-06)
B Abomoelak et al.
Infection and immunity, 67(10), 5100-5105 (1999-09-25)
The development of combined vaccines constitutes one of the priorities in modern vaccine research. One of the most successful combined vaccines in use is the diphtheria-pertussis-tetanus vaccine. However, concerns about the safety of the pertussis arm have led to decreased
Greg Davis et al.
PloS one, 16(7), e0254667-e0254667 (2021-07-15)
The world is currently in a pandemic of COVID-19 (Coronavirus disease-2019) caused by a novel positive-sense, single-stranded RNA β-coronavirus referred to as SARS-CoV-2. Here we investigated rates of SARS-CoV-2 infection in the greater Cincinnati, Ohio, USA metropolitan area from August
Ying-Feng Chang et al.
Biosensors & bioelectronics, 26(3), 1068-1073 (2010-09-22)
Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed
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