P4656
Pepsinogen from porcine stomach
Grade I-S, lyophilized powder, ~3,000 units/mg protein (after activation to pepsin at pH 2.0 at 25°C)
Synonym(s):
Pepsinogen from hog stomach
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About This Item
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biological source
Porcine stomach
type
Grade I-S
Assay
≥85% (biuret)
form
lyophilized powder
specific activity
~3,000 units/mg protein (after activation to pepsin at pH 2.0 at 25°C)
purified by
chromatography
UniProt accession no.
foreign activity
pepsin, free
storage temp.
2-8°C
Gene Information
pig ... PGA5(396892)
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Biochem/physiol Actions
Pepsinogen is the zymogen of pepsin. It is processed by autocatalytic cleavage of 44 amino acids to generate active pepsin. Serum levels of pepsinogen have been measured to identify gastric cancer risk.
Unit Definition
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products, using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
Analysis Note
Protein determined by biuret.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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[Judgment of eradication of H. pylori based on percent changes in serum pepsinogens].
Takahisa Furuta et al.
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology, 110(2), 210-217 (2013-02-06)
Michihiko Sone et al.
Acta oto-laryngologica, 133(1), 35-41 (2012-09-21)
The present study showed that elevation of bone-conduction (BC) thresholds at low frequencies might be a characteristic audiometric finding in cases with otitis media with effusion (OME) with high pepsinogen (PG) concentrations. The objective of this study was to investigate
Serological assessment of samples from patients complaining of dyspepsia.
S Mortlock et al.
British journal of biomedical science, 69(4), 180-182 (2013-01-12)
S V German et al.
Gigiena i sanitariia, (6)(6), 85-87 (2013-03-06)
Assessment of health status of the population - the most important issue in preventive medicine. The objective of this work - to determine the possibility of nonendoscopic screening for gastroduodenal pathology, by the example of atrophic gastritis, in mass medical
Ashraf Mohamadkhani et al.
Archives of Iranian medicine, 16(4), 208-212 (2013-03-19)
Northern Iran (Ardabil) is characterized by a high gastric cancer (GC) rate, whereas Southern Iran (Kerman and Yazd) has a low GC rate. The aim of this study is to verify the potential for pepsinogen I and II to detect
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