P4385
Calmodulin−Agarose
saline suspension
Synonym(s):
Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-Agarose from bovine testes
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About This Item
UNSPSC Code:
41106500
MDL number:
form
saline suspension
extent of labeling
≥0.5 mg per mL
matrix
4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
storage temp.
2-8°C
Application
Calmodulin-agarose is used in affinity chromatography, protein chromatography, calcium associated proteins, and specialty resins. Calmodulin-agarose has been used to suggest a coordinated regulation of processes in both mitochondria and chloroplasts by calcium signals. Calmodulin-agarose has also been used to study apoptosis in prostate cancer cells and to analyze the interaction of the influenza virus polymerase complex with human cell factors.
Physical form
Suspension in 0.5 M NaCl containing 0.02% thimerosal
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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A Takasaki et al.
The Journal of biological chemistry, 274(17), 11848-11853 (1999-04-17)
Various proteins in the signal transduction pathways as well as those of viral origin have been shown to be myristoylated. Although the modification is often essential for the proper functioning of the modified protein, the mechanism by which the modification
M S Rogers et al.
Neoplasia (New York, N.Y.), 1(3), 220-225 (2000-08-10)
Cell proliferation requires calmodulin, a protein that regulates calcium-dependent enzymes involved in signal transduction pathways in eukaryotic cells. Calmodulin-like protein (CLP) is found in certain epithelial cell types, including normal breast epithelium, and, although it closely resembles calmodulin in amino
L Luoni et al.
FEBS letters, 482(3), 225-230 (2000-10-12)
The plasma membrane Ca(2+)-ATPase was purified from Arabidopsis thaliana cultured cells by calmodulin (CaM)-affinity chromatography and reconstituted in proteoliposomes by the freeze-thaw sonication procedure. The reconstituted enzyme catalyzed CaM-stimulated 45Ca(2+) accumulation and H(+) ejection, monitored by the increase of fluorescence
Daisuke Takezawa et al.
Biochemical and biophysical research communications, 317(2), 428-436 (2004-04-06)
Plant responses to environmental stresses are mediated in part by signaling processes involving cytosolic Ca2+ and a Ca(2+)-binding protein, calmodulin. Screening with radiolabeled calmodulin of a cDNA library of the moss Physcomitrella patens resulted in identification of genes encoding novel
S J Lee et al.
Neuron, 32(6), 1097-1106 (2002-01-05)
Hundreds of G protein-coupled receptors (GPCRs) and at least six GPCR kinases have been identified, but the only GPCR phosphatase that has been definitively demonstrated is the rhodopsin phosphatase encoded by the rdgC locus of Drosophila. Mutations in rdgC result
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