P4244
Phosphate buffered saline
liquid, pH 7.2 (25 °C), autoclaved
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Synonym(s):
PBS
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Quality Level
description
Sodium chloride, 150 mM, and sodium phosphate, 150 mM
form
liquid
pH
7.2 (25 °C)
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
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General description
Phosphate buffered saline (PBS) is an isotonic standard buffer solution. PBS imitates the pH, ion concentrations, and osmolarity of the human body. It serves as an ideal buffer for maintaining the osmolarity and physiological pH for biomedical dilutions in cell culture and protein chemistry. PBS is used for standard enzymatic tests with active metal-organic framework (MOF) biocomposites. PBS is considered non-toxic to cells.
Application
Phosphate buffered saline has been used:
- for washing bacterial cells after harvesting by centrifugation
- for washing the peritoneal cavity for isolation of peritoneal macrophages
- as a control and to dilute phytohemagglutinin (PHA) to prepare subcutaneous injections and study the immune response in birds.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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