P4056
Platelet-Derived Growth Factor-BB from rat
PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
PDGF-BB
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About This Item
biological source
rat
Quality Level
recombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
1.62 ng/mL EC50
quality
endotoxin tested
mol wt
~30 kDa
packaging
pkg of 5x50 μg
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Pdgfb(24628)
Related Categories
General description
Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.
Application
Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.
Biochem/physiol Actions
Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
Other Notes
Dimer of the B chain of rat PDGF.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Eileen M Bauer et al.
PloS one, 9(5), e96720-e96720 (2014-05-20)
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Platelet-Derived Growth Factor?BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells.
Millette E, et al.
Trends in Cardiovascular Medicine, 16.1, 25-28 (2006)
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Current protocols, 2(5), e416-e416 (2022-05-10)
The serine/threonine protein kinase Akt integrates diverse upstream inputs to regulate cell survival, growth, metabolism, migration, and differentiation. Mounting evidence suggests that Akt activity is differentially regulated depending on its subcellular location, which can include the plasma membrane, endomembrane, and
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The role of growth factors in wound healing.
David L, et al.
The Surgical Clinics of North America, 77.3, 575-586 (1997)
Articles
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
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