P4020
Protamine sulfate
amorphous powder
Synonym(s):
Salmine, Salmine from salmon
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About This Item
product name
Protamine sulfate salt from salmon, Grade X, amorphous powder
type
Grade X
Quality Level
form
amorphous powder
impurities
histone, free (Millon test)
color
white to off-white
solubility
6 M HCl: 25 mg/mL
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General description
Protamine are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. It is found to be localized to the sperm head.
Application
Protamine sulfate salt from salmon has been used:
- in the pretreament of cells to study its effect on respiratory syncytial virus infection
- in lentivirus production
- to derivatize it into a diazeniumdiolate NO donor
May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Biochem/physiol Actions
Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
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Steve N Bailey et al.
Nature methods, 3(2), 117-122 (2006-01-25)
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin
The design and evaluation of heparin-binding foldamers.
Sungwook Choi et al.
Angewandte Chemie (International ed. in English), 44(41), 6685-6689 (2005-08-12)
Microarrays of lentiviruses for gene function screens in immortalized and primary cells
Bailey SN, et al.
Nature Methods, 3(2), 117-117 (2006)
Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection
Hallak LK, et al.
Virology, 271(2), 264-275 (2000)
Diazeniumdiolation of protamine sulfate reverses mitogenic effects on smooth muscle cells and fibroblasts
Van Lith R, et al.
Free radical biology & medicine, 82, 13-21 (2015)
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