P3775
Anti-Protein A antibody produced in rabbit
fractionated antiserum, lyophilized powder
Synonym(s):
Anti-Protein A
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
packaging
vial of 2 mL lyophilized antiserum
technique(s)
indirect ELISA: 1:100,000
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.
Immunogen
Protein A from Staphylococcus aureus
Application
Anti-Protein A antibody produced in rabbit has been used in immunoblotting.
Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.
Biochem/physiol Actions
Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.
Physical form
Lyophilized from 0.01M phosphate buffered saline, pH 7.2
Reconstitution
Reconstitute with 2 ml deionized water.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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control
Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Clement ST, et al.
Science Signaling, 6(291), ra78-ra78 (2013)
Shintaro Kira et al.
Autophagy, 10(9), 1565-1578 (2014-07-22)
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Smriti Kala et al.
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IMMUNOASSAY CONFIGURATIONS
Immunoassay (1996)
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