P3752
Phosphatase, Acid from potato
lyophilized powder, ≥0.5 unit/mg solid
Synonym(s):
(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
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About This Item
CAS Number:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
Specific activity:
≥0.5 unit/mg solid
Biological source:
potato
biological source
potato
Quality Level
form
lyophilized powder
specific activity
≥0.5 unit/mg solid
mol wt
69 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
Application
Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.
Biochem/physiol Actions
Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Protocols
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
Related Content
K Zyła et al.
The British journal of nutrition, 74(1), 3-17 (1995-07-01)
An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4)
Jia Zhang et al.
Biosensors & bioelectronics, 141, 111442-111442 (2019-06-30)
Phenol contains an exchangeable hydroxyl proton resonant at 4.8 ppm from the resonance frequency of water in the 1H nuclear magnetic resonance (1H NMR) spectrum, enabling itself to be detected at sub-mM concentration by either chemical exchange saturation transfer magnetic resonance
Filipe Natalio et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 23(21), 4973-4980 (2017-03-02)
Boron's unusual properties inspired major advances in chemistry. In nature, the existence and importance of boron has been fairly explored (e.g. bacterial signaling, plant development) but its role as biological catalyst was never reported. Here, we show that boric acid
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3752-1G | 04061835506552 |
| P3752-100MG | 04061834372837 |