P354-05
Porcine Aortic Smooth Muscle Cells: PAOSMC (Cryovial)
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
biological source
Porcine aorta (normal)
Quality Level
form
solid
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 38
morphology
smooth muscle
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
Related Categories
General description
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PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
Cell Line Origin
Aorta
Application
aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation
Components
Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
Preparation Note
- 1st passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
Subculture Routine
Please refer to the PAOSMC Culture Protocol.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Nina Parmar et al.
Tissue engineering. Part C, Methods, 21(4), 404-412 (2014-09-11)
Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are
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