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P3303

Asp-N Protease

Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain

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About This Item

CAS Number:
9001-92-7
EC Number:
3.4.24.33 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.56
EC Number:
232-642-4
MDL number:
MFCD02253021
eCl@ss:
32160410
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Product Name

Endoproteinase Asp-N from Pseudomonas fragi mutant strain, suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

Quality Level

200

form

lyophilized powder

analyte chemical class(es)

amino acids

packaging

vial of 2 μg

suitability

suitable for protein sequencing

storage temp.

2-8°C

General description

Endoproteinase Asp-N is a metallo endoprotease. It is obtained from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues. Asp-N is used in proteomics for peptide mapping and protein sequence work due to its highly specific cleavage of peptides.

Application

Endoproteinase Asp-N from Pseudomonas fragi mutant strain has been used for the digestion of specific proteins to prepare peptides and for the analysis of generated peptides by MS (mass spectrometry) method.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000500467
0000500467
0000498468
0000498468
0000474316

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Protocols

Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

阿达木单抗（adalimumab）肽图谱 LC-MS/MS 分析工作流程

基于优化的 LC-MS/MS 工作流程，通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。

Articles

Peptide Mapping of Trastuzumab for Biosimilar Comparability

Comprehensive LC-MS peptide mapping method for biosimilar comparability of trastuzumab, enabling full sequence coverage and PTM analysis.

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Global Trade Item Number

SKUGTIN
P3303-1VL04061834367659